| Gene name |
SPBC83.14c |
| Gene ID |
20/E03 |
| Gene synonyms/obsolete |
rfc5 |
| Gene product |
replication factor C
(activator 1 subunit); AAA family ATPase; activator of DNA
polymerases |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1280 |
| ORF length (spliced) |
1077 |
| Entry clone length |
1280 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1215T:C /
1268A:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC83.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTGGTTAGATCAATA |
| Rev primer name |
SPBC83.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAAGAAGGTAACTCCATA |
| Amino acid length |
358 |
| Molecular weight |
40.4 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol
|
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |