| Gene name |
SPAC24H6.12c |
| Gene ID |
20/F06 |
| Gene synonyms/obsolete |
uba3 |
| Gene product |
ubiquitin activating
enzyme |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1335 |
| ORF length (spliced) |
|
| Entry clone length |
1335 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
99A:G / 336T:C /
501C:T / 820A:G / 1287T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC24H6.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCTCCTCAGACGTATG |
| Rev primer name |
SPAC24H6.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCATTCTCTCAACTGAACA |
| Amino acid length |
444 |
| Molecular weight |
48.9 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGCEILKDLAL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |