| Gene name |
SPAC144.17c |
| Gene ID |
20/H02 |
| Gene synonyms/obsolete |
|
| Gene product |
6-phosphofructo-2-kinase;
fructose-2,6-bisphosphatase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1349 |
| ORF length (spliced) |
1299 |
| Entry clone length |
1349 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
358A:G / 949T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC144.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGCGCAGAAATTCAAT |
| Rev primer name |
SPAC144.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTGTAAGTTGCAATGGAC |
| Amino acid length |
432 |
| Molecular weight |
50.2 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAVRLEPLIL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |