Gene name |
SPCC550.12 |
Gene ID |
20/H04 |
Gene synonyms/obsolete |
|
Gene product |
actin-like
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1349 |
ORF length (spliced) |
1206 |
Entry clone length |
1349 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
131T:deletion /
591T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC550.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGACTACCCCCTAAAAC |
Rev primer name |
SPCC550.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATTCTCCTTTTTCGTGTC |
Amino acid length |
401 |
Molecular weight |
45.8 |
Isoelectric point (calc.) |
5.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |