Gene name |
SPAC821.11 |
Gene ID |
21/A06 |
Gene synonyms/obsolete |
pro1 |
Gene product |
gamma-glutamyl
phosphate reductase |
Entry clone |
Cloned |
ORF length (unspliced) |
1356 |
ORF length (spliced) |
|
Entry clone length |
1356 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
400A:G / 635A:G /
657T:C / 912T:C / 1311T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC821.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCTTGAAGAGAATGT |
Rev primer name |
SPAC821.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTTCGAAACCTGAGAA |
Amino acid length |
451 |
Molecular weight |
48.7 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDKIVEELRI/LSSSMVKRLDL |
Localization (YFP) |
cytosol>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
Microscope used for
observation |
DeltaVision |