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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6F6.12
Gene ID 21/B04
Gene synonyms/obsolete
Gene product sorting nexin; involved in proteasome function; similar to Sp SPBC1711.11; PX domain; phosphoinositide binding; involved in intracellular protein transport
Entry clone Cloned
ORF length (unspliced) 1362
ORF length (spliced) 1206
Entry clone length 1362
No. of intron 2
Sequence status Finished
Sequence results 314T:C / 668A:G / 1131T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6F6.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGATTCTGTGAATTT
Rev primer name SPAC6F6.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTGCTTAACACGTATCCAA
Amino acid length 401
Molecular weight 46.3
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEGSIQKLLRL
Localization (YFP) Golgi
Comments for localization large cytoplasmic dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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