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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4F8.07c
Gene ID 21/B10
Gene synonyms/obsolete hxk2
Gene product hexokinase 2
Entry clone Cloned
ORF length (unspliced) 1368
ORF length (spliced)
Entry clone length 1368
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4F8.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGCTAATTTTCAACA
Rev primer name SPAC4F8.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGGCCAAGCCTTTCTTTTCC
Amino acid length 455
Molecular weight 50.9
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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