Gene name |
SPCC1827.06c |
Gene ID |
21/C12 |
Gene synonyms/obsolete |
|
Gene product |
aspartate semialdehyde
dehydrogenase; involved in threonine biosynthesis; involved in
methionine metabolism; aspartate-semialdehyde dehydrogenase
activity |
Entry clone |
Cloned |
ORF length (unspliced) |
1378 |
ORF length (spliced) |
1074 |
Entry clone length |
1378 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1827.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTATAAAAAAAGTTGG |
Rev primer name |
SPCC1827.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATAAGTCCCTTCTTCACC |
Amino acid length |
357 |
Molecular weight |
38.5 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |