Gene name |
SPBC1711.08 |
Gene ID |
21/D12 |
Gene synonyms/obsolete |
|
Gene product |
stress-regulated
co-chaperone; activator of Hsp90p; interacts physically with
Swo1p; conserved eukaryotic protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1386 |
ORF length (spliced) |
1011 |
Entry clone length |
1386 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
481T:C / 747T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1711.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGCCACATCTATAAA |
Rev primer name |
SPBC1711.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAGAACAGCGCCAAAACCA |
Amino acid length |
336 |
Molecular weight |
37.4 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
moving dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |