Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC4.06
Gene ID 21/E02
Gene synonyms/obsolete
Gene product acid phosphatase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1389
ORF length (spliced)
Entry clone length 1389
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC4.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCAAATGTCTTCAGC
Rev primer name SPBC4.06.Rv
Rev primer SEQ AGAAAGCTGGGTACTTTTTCACCGGTTTACAC
Amino acid length 462
Molecular weight 51.9
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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