Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4F10.18
Gene ID 21/F03
Gene synonyms/obsolete
Gene product conserved hypothetical protein; WD repeat protein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1396
ORF length (spliced) 1176
Entry clone length 1396
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC4F10.18.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTCTTTCCTCTAACCA
Rev primer name SPAC4F10.18.Rv
Rev primer SEQ AGAAAGCTGGGTATAATCTCGTAAACCCCATC
Amino acid length 391
Molecular weight 42.7
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLTLNTLPL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.