Gene name |
SPBC23E6.05 |
Gene ID |
21/H11 |
Gene synonyms/obsolete |
|
Gene product |
metallopeptidase;
peptidase family M24; involved in ribosome biogenesis
(predicted) |
Entry clone |
Cloned |
ORF length (unspliced) |
1427 |
ORF length (spliced) |
1254 |
Entry clone length |
1427 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
937A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC23E6.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGCTCGATCCTTCTGA |
Rev primer name |
SPBC23E6.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACGTTAATGTTTATGCTT |
Amino acid length |
417 |
Molecular weight |
45.6 |
Isoelectric point (calc.) |
6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol; nuclear envelope by over
expression |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |