Gene name |
SPAC1F12.06c |
Gene ID |
22/A11 |
Gene synonyms/obsolete |
|
Gene product |
endonuclease; no Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
845 |
ORF length (spliced) |
759 |
Entry clone length |
845 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
400T:C / 766G:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1F12.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAATGACAATTTGCA |
Rev primer name |
SPAC1F12.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTTTTCTTATTCCTTTTC |
Amino acid length |
252 |
Molecular weight |
28.7 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LACHLGVLLNL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |