| Gene name |
SPAC22A12.10 |
| Gene ID |
22/B04 |
| Gene synonyms/obsolete |
|
| Gene product |
aminoalcoholphosphotransferase; involved in
phosphatidylethanolamine biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1211 |
| ORF length (spliced) |
1161 |
| Entry clone length |
1211 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
457T:C / 944T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22A12.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGTTAAACCGGAAGCA |
| Rev primer name |
SPAC22A12.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTCTTCTTGGACTCTGGT |
| Amino acid length |
386 |
| Molecular weight |
44 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLTIELLQL/LYNFMAFALLL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |