Gene name |
SPBC19G7.16 |
Gene ID |
22/C10 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein; predicted coiled-coil region |
Entry clone |
Cloned |
ORF length (unspliced) |
1287 |
ORF length (spliced) |
|
Entry clone length |
1287 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
308A:G / 1064A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19G7.16.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAAGAAGAAAAGGC |
Rev primer name |
SPBC19G7.16.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGTCCACGACCTTCAATA |
Amino acid length |
428 |
Molecular weight |
49.1 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMDILTKLPI |
Localization (YFP) |
nucleolus>nucleus;
spindle microtubules |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus) |
Microscope used for
observation |
Confocal |