| Gene name |
SPBC428.11 |
| Gene ID |
22/D02 |
| Gene synonyms/obsolete |
|
| Gene product |
homocysteine synthase;
non-essential; involved in sulfur metabolism
(regulation) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1290 |
| ORF length (spliced) |
|
| Entry clone length |
1290 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
(-12)G:deletion /
851T:C |
| Comments |
ApaI or SacII should
be used instead of NotI for integration using pDUAL. 5'
terminus is frameshifted. |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC428.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAGTCGAGAGTGAACA |
| Rev primer name |
SPBC428.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGCAACTTCCAAAGCTTGA |
| Amino acid length |
429 |
| Molecular weight |
46.4 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLQGLETLSL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |