| Gene name |
SPAC23A1.02c |
| Gene ID |
22/D11 |
| Gene synonyms/obsolete |
|
| Gene product |
calcineurin-like
phosphoesterase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1293 |
| ORF length (spliced) |
|
| Entry clone length |
1293 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23A1.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGCAAGACATCCGAA |
| Rev primer name |
SPAC23A1.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGATATGTTGCAATGATATT |
| Amino acid length |
430 |
| Molecular weight |
48.6 |
| Isoelectric point (calc.) |
7.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLWLNKQLSI/LFGIMPRKLQL/LHISVCVLTI |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |