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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F5.10
Gene ID 22/F09
Gene synonyms/obsolete tif412
Gene product translation initiation factor (4a subunit); DEAD/DEAH box helicase; similar to Sp SPAC1006.07
Entry clone Cloned
ORF length (unspliced) 1304
ORF length (spliced) 1185
Entry clone length 1304
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F5.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGGACGAAATAATGGA
Rev primer name SPAC1F5.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAACCATATCTCCTATATTC
Amino acid length 394
Molecular weight 44.7
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LCDLYDTLTI
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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