Gene name |
SPAC3G9.07c |
Gene ID |
22/F12 |
Gene synonyms/obsolete |
phd1; hda1 |
Gene product |
transcriptional
regulator; histone deacetylase; NAD-independent histone
deacetylase activity; RPD3-like (class I); involved in
transcriptional regulation; involved in chromatin silencing;
involved in chromatin assembly/disassembly |
Entry clone |
Cloned |
ORF length (unspliced) |
1305 |
ORF length (spliced) |
|
Entry clone length |
1305 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3G9.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATACTCCTGAGACATC |
Rev primer name |
SPAC3G9.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCCTCGAACGCGAACATCC |
Amino acid length |
434 |
Molecular weight |
49.3 |
Isoelectric point (calc.) |
5.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRFFPRVLYI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |