Gene name |
SPAC1002.14 |
Gene ID |
22/G04 |
Gene synonyms/obsolete |
itt1 |
Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); IBR
domains; inhibitor of translation termination |
Entry clone |
Cloned |
ORF length (unspliced) |
1308 |
ORF length (spliced) |
|
Entry clone length |
1308 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
883T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1002.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGGAGGTGGAGGTGGA |
Rev primer name |
SPAC1002.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTCCAGTTTTCCGAAAAA |
Amino acid length |
435 |
Molecular weight |
50.2 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |