Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC417.11c
Gene ID 22/G06
Gene synonyms/obsolete
Gene product putative glutamate-1-semialdehyde aminotransferase; no Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1308
ORF length (spliced)
Entry clone length 1308
No. of intron 0
Sequence status Finished
Sequence results 408A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC417.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTTCTGAGATATCGGA
Rev primer name SPCC417.11.Rv
Rev primer SEQ AGAAAGCTGGGTATATTCTAATAAGAGACATC
Amino acid length 435
Molecular weight 47.6
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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