Gene name |
SPBC106.04 |
Gene ID |
22/G11 |
Gene synonyms/obsolete |
ada1 |
Gene product |
adenosine OR AMP
deaminase activity; involved in purine metabolism;
non-essential |
Entry clone |
Cloned |
ORF length (unspliced) |
2721 |
ORF length (spliced) |
2541 |
Entry clone length |
2721 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
62C:T / 162A:G /
548T:C / 938A:G / 1930A:G / 2568T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC106.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTACGTCCTCTCTC |
Rev primer name |
SPBC106.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTTTCAAATCTTGGCTA |
Amino acid length |
846 |
Molecular weight |
97.4 |
Isoelectric point (calc.) |
6.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
829 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQEVFDSLKL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |