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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H3.03c
Gene ID 22/H04
Gene synonyms/obsolete
Gene product similar to nitrogen permease regulator; human ortholog is a candidate tumour suppressor; involved in cysplatin resistance
Entry clone Cloned
ORF length (unspliced) 1314
ORF length (spliced) 1230
Entry clone length 1314
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H3.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGTATTCTGAAGAGGG
Rev primer name SPAC23H3.03.Rv
Rev primer SEQ AGAAAGCTGGGTATACATAAATAAAACAGGCA
Amino acid length 409
Molecular weight 47
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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