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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G7.10
Gene ID 22/H12
Gene synonyms/obsolete
Gene product Myb family; DNA binding protein
Entry clone Cloned
ORF length (unspliced) 1318
ORF length (spliced) 1173
Entry clone length 1318
No. of intron 2
Sequence status Finished
Sequence results 50A:G / 497A:T / 1276T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC13G7.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGGTCTATGAAACCTCC
Rev primer name SPAC13G7.10.Rv
Rev primer SEQ AGAAAGCTGGGTATCCCCGGTTGTCCCACGGT
Amino acid length 390
Molecular weight 43.9
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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