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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC31H12.06
Gene ID 23/B02
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1407
ORF length (spliced)
Entry clone length 1407
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC31H12.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTAAGCAAAAAAATGAA
Rev primer name SPCC31H12.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGGTAGCCCAGTAAAAGA
Amino acid length 468
Molecular weight 52.6
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLLRVSLLQL/LLGSISSYLLL
Localization (YFP) aggregates of ambiguous structure by over expression
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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