| Gene name |
SPAC27E2.05 |
| Gene ID |
23/B11 |
| Gene synonyms/obsolete |
cdc1; mis1 |
| Gene product |
DNA polymerase delta
(small subunit); interacts physically with Cdc27p; interacts
physically with Pol3p; involved in DNA replication (required);
involved in cell cycle progression (required); involved in
telomere maintenance |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1438 |
| ORF length (spliced) |
1389 |
| Entry clone length |
1438 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
339A:G / 1323T:C /
1340A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27E2.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTGAAGTCAGACATTC |
| Rev primer name |
SPAC27E2.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAAGTAATTGGAGTCTCT |
| Amino acid length |
462 |
| Molecular weight |
51.3 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |