| Gene name |
SPBC215.03c |
| Gene ID |
23/C05 |
| Gene synonyms/obsolete |
csn1 |
| Gene product |
COP9/signalosome
complex (subunit 1); required for the removal of Ned8p from
Pcu1p; involved in DNA damage checkpoint; no apparent Sc
ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1443 |
| ORF length (spliced) |
1269 |
| Entry clone length |
1443 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
180T:C / 1167T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC215.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCTTAAATCTACTAGT |
| Rev primer name |
SPBC215.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTCTGTGAATCTTCCGCG |
| Amino acid length |
422 |
| Molecular weight |
48.6 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |