| Gene name |
SPCC290.01c |
| Gene ID |
23/D04 |
| Gene synonyms/obsolete |
fum1; SPCC18.18c |
| Gene product |
fumarate hydratase
(predicted); involved in fumarate metabolism and TCA
cycle |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1449 |
| ORF length (spliced) |
|
| Entry clone length |
1449 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
746T:C / 1238A:G /
1310A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC290.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTCCACTACTCCGAC |
| Rev primer name |
SPCC290.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACTTTCTTAGCTGAAATC |
| Amino acid length |
482 |
| Molecular weight |
52.2 |
| Isoelectric point (calc.) |
7.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
bright signal |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |