Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC27F1.05c
Gene ID 23/E01
Gene synonyms/obsolete
Gene product aminotransferase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1455
ORF length (spliced)
Entry clone length 1455
No. of intron 0
Sequence status Finished
Sequence results 715T:C / 1095T:C / 1442C:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC27F1.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTGATTCCACGTCCAC
Rev primer name SPAC27F1.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGGTTGACTTGGCTGCAACA
Amino acid length 484
Molecular weight 53.1
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots; cytosol
Comments for localization aggregates by over expression; moving dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.