Gene name |
SPAC27F1.05c |
Gene ID |
23/E01 |
Gene synonyms/obsolete |
|
Gene product |
aminotransferase; no
apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1455 |
ORF length (spliced) |
|
Entry clone length |
1455 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
715T:C / 1095T:C /
1442C:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC27F1.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGATTCCACGTCCAC |
Rev primer name |
SPAC27F1.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTTGACTTGGCTGCAACA |
Amino acid length |
484 |
Molecular weight |
53.1 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
cytosol |
Comments for localization |
aggregates by over
expression; moving dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |