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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC56F8.05c
Gene ID 23/G07
Gene synonyms/obsolete
Gene product conserved hypothetical protein; conserved yeast protein; similar to S. cerevisiae YIL041W
Entry clone Cloned
ORF length (unspliced) 1474
ORF length (spliced) 888
Entry clone length 1474
No. of intron 5
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC56F8.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTATTCGACATTGGTAAA
Rev primer name SPAC56F8.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGATGGTTATATCCGATAGA
Amino acid length 295
Molecular weight 32.7
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization cytoplasmic dots, especially near site of septum formation
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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