| Gene name |
SPCC70.03c |
| Gene ID |
23/H05 |
| Gene synonyms/obsolete |
|
| Gene product |
proline dehydrogenase;
involved in proline catabolism; involved in glutamate
biosynthesis; proline dehydrogenase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1479 |
| ORF length (spliced) |
|
| Entry clone length |
1479 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
609T:C / 812C:T /
1347T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC70.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTGCCTTTCGCCTTGC |
| Rev primer name |
SPCC70.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATATACGACGACGAAGA |
| Amino acid length |
492 |
| Molecular weight |
55.8 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTPFINPLVL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |