Gene name |
SPAC23C4.14 |
Gene ID |
23/H11 |
Gene synonyms/obsolete |
|
Gene product |
beta-mannosyltransferase; involved in
N-glycosylation |
Entry clone |
Cloned |
ORF length (unspliced) |
1485 |
ORF length (spliced) |
1275 |
Entry clone length |
1485 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23C4.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTAGTATTGAAGATAGT |
Rev primer name |
SPAC23C4.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTTGAACCTTGAACTATA |
Amino acid length |
424 |
Molecular weight |
48.7 |
Isoelectric point (calc.) |
9.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVLKIVLFLSL/LKVLHQFLAL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |