Gene name |
SPCC548.01 |
Gene ID |
24/A01 |
Gene synonyms/obsolete |
dga1;
SPCC1235.15 |
Gene product |
diacylglycerol
acyltransferase |
Entry clone |
Cloned |
ORF length (unspliced) |
1487 |
ORF length (spliced) |
1050 |
Entry clone length |
1487 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC548.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAAGAAACATCAAT |
Rev primer name |
SPCC548.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCTGACAACTTCAATTCG |
Amino acid length |
349 |
Molecular weight |
39.9 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPNRISELKL |
Localization (YFP) |
ER; cytoplasmic
dots |
Comments for localization |
large aggregates
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |