Gene name |
SPAC13A11.02c |
Gene ID |
24/A02 |
Gene synonyms/obsolete |
cyp51 |
Gene product |
probable cytochrome
P450 51 |
Entry clone |
Cloned |
ORF length (unspliced) |
1488 |
ORF length (spliced) |
|
Entry clone length |
1488 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13A11.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATTCTCTTTAGTTTC |
Rev primer name |
SPAC13A11.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTACGACGCTTCCAAGCA |
Amino acid length |
495 |
Molecular weight |
56.3 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNYVIQETLRL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |