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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC821.07c
Gene ID 24/B03
Gene synonyms/obsolete
Gene product zf-fungal Zn(2)-Cys(6) binuclear cluster domain; transcriptional regulator
Entry clone Cloned
ORF length (unspliced) 1494
ORF length (spliced)
Entry clone length 1494
No. of intron 0
Sequence status Finished
Sequence results 501T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC821.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAACGGAATAGAACGGG
Rev primer name SPAC821.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTACTGGTCGATTTAAG
Amino acid length 497
Molecular weight 55.3
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 24/28
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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