| Gene name |
SPAC16C9.03 |
| Gene ID |
24/B11 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in mRNA
catabolism, nonsense-mediated |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1497 |
| ORF length (spliced) |
|
| Entry clone length |
1497 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
237A:G / 787T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16C9.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATATGGACAGTGGAAG |
| Rev primer name |
SPAC16C9.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATATGCATTGCCTCAACG |
| Amino acid length |
498 |
| Molecular weight |
56.5 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol (intranuclear microtubule
bundle?) |
| Microscope used for
observation |
Confocal |