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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC637.13c
Gene ID 24/C03
Gene synonyms/obsolete
Gene product hypothetical protein; pleckstrin homology domain
Entry clone Cloned
ORF length (unspliced) 1497
ORF length (spliced)
Entry clone length 1497
No. of intron 0
Sequence status Finished
Sequence results 820C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC637.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACTTTCAGGGAAATC
Rev primer name SPAC637.13.Rv
Rev primer SEQ AGAAAGCTGGGTATACATTCCAAGCATTATGA
Amino acid length 498
Molecular weight 56
Isoelectric point (calc.) 6.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPGTIINILEL
Localization (YFP) periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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