| Gene name |
SPBC21D10.11c |
| Gene ID |
24/C05 |
| Gene synonyms/obsolete |
|
| Gene product |
aminotranferase class
V; cysteine desulpherase; involved in cysteine metabolism;
involved in alanine metabolism; possibly supplies inorganic
sulfur for iron sulfur clusters; (old->tRNA splicing
protein) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1497 |
| ORF length (spliced) |
|
| Entry clone length |
1497 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
214C:T / 521A:G /
1214A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC21D10.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAAGTTTGATGCTTTC |
| Rev primer name |
SPBC21D10.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGTTGACTCCATTCTATA |
| Amino acid length |
498 |
| Molecular weight |
55.1 |
| Isoelectric point (calc.) |
7.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
312 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal |