Gene name |
SPCC1620.02 |
Gene ID |
24/C07 |
Gene synonyms/obsolete |
wtf23; wtf10 |
Gene product |
wtf element |
Entry clone |
Cloned |
ORF length (unspliced) |
1498 |
ORF length (spliced) |
1107 |
Entry clone length |
1498 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
145T:C / 1322T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1620.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAATAAATATTACCC |
Rev primer name |
SPCC1620.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACTTCGCCTTCGACATCC |
Amino acid length |
368 |
Molecular weight |
41.1 |
Isoelectric point (calc.) |
5 |
Signal SEQ |
|
No. of transmembrane domain |
7 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFIMGNVLFL |
Localization (YFP) |
Golgi? |
Comments for localization |
large aggregates by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |