| Gene name |
SPBC577.09 |
| Gene ID |
24/C11 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat protein;
highly similar to human cockayne syndrome WD repeat protein;
no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1500 |
| ORF length (spliced) |
1215 |
| Entry clone length |
1500 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
704C:T / 1108A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC577.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTCTTTTTTATTGGC |
| Rev primer name |
SPBC577.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGATAAGTCACTGGAATA |
| Amino acid length |
404 |
| Molecular weight |
45.5 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQHRLQRLAL/LGSVNALSI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |