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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC725.14
Gene ID 24/D03
Gene synonyms/obsolete
Gene product acetylglutamate synthase; involved in ornithine biosynthesis; involved in arginine biosynthesis; amino-acid N-acetyltransferase activity
Entry clone Cloned
ORF length (unspliced) 1503
ORF length (spliced)
Entry clone length 1503
No. of intron 0
Sequence status Finished
Sequence results 623A:G / 634T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC725.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAAAACCTTCATTGTC
Rev primer name SPBC725.14.Rv
Rev primer SEQ AGAAAGCTGGGTAATTCAACCATGTTGGCTGA
Amino acid length 500
Molecular weight 55.7
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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