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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC550.10
Gene ID 24/D05
Gene synonyms/obsolete meu8
Gene product betaine aldehyde dehydrogenase; involved in betaine biosynthesis from choline; involved in meiosis; betaine-aldehyde dehydrogenase activity; meiotic expression upregulated; no apparent Sc ortholog
Entry clone Cloned in 2006 trial
ORF length (unspliced) 1503
ORF length (spliced)
Entry clone length 1503
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC550.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTATAGATTTAAATGT
Rev primer name SPCC550.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGCCAACATACTGAGTGATT
Amino acid length 500
Molecular weight 54.1
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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