Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8C9.03
Gene ID 24/D06
Gene synonyms/obsolete cgs1; rak1
Gene product cAMP-dependent protein kinase; negative regulator of meiosis; mutant (cgs1-1) displays cAPK activity unregulated by cyclic AMP (cAMP); (old-> cAMP-dependent protein kinase regulatory chain)
Entry clone Cloned#
ORF length (unspliced) 1504
ORF length (spliced) 1239
Entry clone length 1504
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC8C9.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTCGAAGAAGTATA
Rev primer name SPAC8C9.03.Rv
Rev primer SEQ AGAAAGCTGGGTATGCTTTAGTTGATGGAGGT
Amino acid length 412
Molecular weight 46.4
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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