| Gene name |
SPAC13G7.08c |
| Gene ID |
24/D08 |
| Gene synonyms/obsolete |
crb3 |
| Gene product |
WD repeat protein; DNA
damage and replication checkpoint control protein;
essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1506 |
| ORF length (spliced) |
1341 |
| Entry clone length |
1506 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC13G7.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGCTGCTTCTTTCTGG |
| Rev primer name |
SPAC13G7.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTTTGCAGGTATTTTTCC |
| Amino acid length |
446 |
| Molecular weight |
49.5 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTVSFDASLLI |
| Localization (YFP) |
SPB; nuclear dots;
nucleus>>cytosol; periphery at site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |