Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC12C2.06
Gene ID 24/E05
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; ATP-dependent; RNA helicase; involved in mRNA expor
Entry clone Cloned
ORF length (unspliced) 1512
ORF length (spliced)
Entry clone length 1512
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC12C2.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAACTACACTGGGACA
Rev primer name SPBC12C2.06.Rv
Rev primer SEQ AGAAAGCTGGGTACATTTTAAGTGCATTCTTG
Amino acid length 503
Molecular weight 56.3
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVELYGLLTI
Localization (YFP) cytosol; nuclear envelope
Comments for localization
Effect of LMB on protein localization changed to: nucleus>cytosol; nuclear envelope
Microscope used for observation Leica

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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