| Gene name |
SPBC30D10.17c |
| Gene ID |
24/E11 |
| Gene synonyms/obsolete |
|
| Gene product |
glucan synthase
regulator; involved in beta-1,3 glucan biosynthesis; involved
in cell wall organization and biogenesis; similar to N. crassa
GS-1 |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1515 |
| ORF length (spliced) |
|
| Entry clone length |
1515 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC30D10.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAAAAATTCGTTTTC |
| Rev primer name |
SPBC30D10.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCTTGCTTCACACTTGTA |
| Amino acid length |
504 |
| Molecular weight |
55.5 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPRDVRESLYI/LFGVTLLDI |
| Localization (YFP) |
periphery; periphery
at site of septum formation |
| Comments for localization |
large aggregates by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |