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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8E11.01c
Gene ID 24/G07
Gene synonyms/obsolete SPAC959.01
Gene product glycosyl hydrolase family 32; invertase
Entry clone Cloned
ORF length (unspliced) 1527
ORF length (spliced)
Entry clone length 1527
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC8E11.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTGTGAGACCATGCAT
Rev primer name SPAC8E11.01.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAATGCGGTCTGTTGTTT
Amino acid length 508
Molecular weight 58
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LESPMEFLCL
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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