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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC26A3.02
Gene ID 24/G10
Gene synonyms/obsolete myh1; myh
Gene product MutY homolog; involved in mismatch repair; interacts physically with Pcn1p; adenine DNA glycosylase; A/8-oxoG glycosylase; deletion mutant has mutator phenotype; oxidative DNA damage protection; no apparent Sc ortholog (old-> A/G-specific adenine DNA glycosylase)
Entry clone Cloned
ORF length (unspliced) 1528
ORF length (spliced) 1386
Entry clone length 1528
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC26A3.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGATTCAAATCATTC
Rev primer name SPAC26A3.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGCACTCTGCTTTCGTTACA
Amino acid length 461
Molecular weight 52.9
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
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