Gene name |
SPAC2G11.06 |
Gene ID |
24/H04 |
Gene synonyms/obsolete |
vps4 |
Gene product |
AAA family ATPase;
involved in intracellular protein transport; involved in
endosomal maturation; class E vps; bam1/bed5 suppressor; MIT
domain |
Entry clone |
Cloned |
ORF length (unspliced) |
1534 |
ORF length (spliced) |
1299 |
Entry clone length |
1534 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC2G11.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCAATCCAGATTGTTT |
Rev primer name |
SPAC2G11.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCCTCTGCTCCAAAGTCC |
Amino acid length |
432 |
Molecular weight |
48.4 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol;
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |