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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2G11.06
Gene ID 24/H04
Gene synonyms/obsolete vps4
Gene product AAA family ATPase; involved in intracellular protein transport; involved in endosomal maturation; class E vps; bam1/bed5 suppressor; MIT domain
Entry clone Cloned
ORF length (unspliced) 1534
ORF length (spliced) 1299
Entry clone length 1534
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2G11.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCAATCCAGATTGTTT
Rev primer name SPAC2G11.06.Rv
Rev primer SEQ AGAAAGCTGGGTATCCCTCTGCTCCAAAGTCC
Amino acid length 432
Molecular weight 48.4
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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