| Gene name |
SPAC1002.09c |
| Gene ID |
24/H06 |
| Gene synonyms/obsolete |
dld1; dldh |
| Gene product |
dihydrolipoamide
dehydrogenase (pyruvate dehydrogenase complex e3 component)
(oxoglutarate dehydrogenase complex e3 component); involved in
glycine catabolism; involved in isoleucine catabolism;
involved in leucine catabolism; involved in serine
biosynthesis; involved in valine catabolism; involved in
acetyl-CoA biosynthesis from pyruvate; involved in G1/S
transition of mitotic cell cycle; involved in tricarboxylic
acid cycle; involved in 2-oxoglutarate metabolism;
dihydrolipoamide dehydrogenase activity; localization pyruvate
dehydrogenase complex (sensu Eukarya); localization
oxoglutarate dehydrogenase complex |
| Entry clone |
Cloned; Mixture |
| ORF length (unspliced) |
1536 |
| ORF length (spliced) |
|
| Entry clone length |
1536 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
check |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1002.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGAACTCTGTGATTAA |
| Rev primer name |
SPAC1002.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGTGAATACTCTTTCCA |
| Amino acid length |
511 |
| Molecular weight |
54.7 |
| Isoelectric point (calc.) |
8.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |